• Genetically modified cropsPosted 2 years ago under Uncategorized

    Genetically modified crops have been shown to disrupt other crops that are not in the same field as the GMOs.

    Genetically modified crops (GMOs, GM crops, or biotech crops) are plants used in agriculture, the DNA of which has been modified using genetic engineering techniques. In most cases the aim is to introduce a new trait to the plant which does not occur naturally in the species. Examples in food crops include resistance to certain pests, diseases, or environmental conditions, reduction of spoilage, or resistance to chemical treatments (e.g. resistance to a herbicide), or improving the nutrient profile of the crop. Examples in non-food crops include production of pharmaceutical agents, biofuels, and other industrially useful goods, as well as for bioremediation.

    Farmers have widely adopted GM technology. Between 1996 and 2011, the total surface area of land cultivated with GM crops had increased by a factor of 94, from 17,000 square kilometers (4,200,000 acres) to 1,600,000 km2 (395 million acres). 10% of the world’s crop lands were planted with GM crops in 2010. As of 2011, 11 different transgenic crops were grown commercially on 395 million acres (160 million hectares) in 29 countries.

    There is broad scientific consensus that food on the market derived from GM crops poses no greater risk to human health than conventional food.12345 It is also said that GM crops provide a number of ecological benefits.6 However, opponents have objected to GM crops per se on several grounds, including environmental concerns, lack or long term testing and safety concerns with health and GM crops, whether GM crops are truly needed to address the world’s food needs, and economic as well as human right concerns raised by the fact these organisms are subject to intellectual property law.

    Gene transfer in nature and traditional agriculture

    Scientists first discovered that DNA naturally transfers between organisms in 1946.7 It is now known that there are several natural mechanisms for flow of genes, or (horizontal gene transfer), and that these occur in nature on a large scale – for example, it is a major mechanism for antibiotic resistance in pathogenic bacteria, and it occurs between plant species.8 This is facilitated by transposons, retrotransposons, proviruses and other mobile genetic elements that naturally translocate to new sites in a genome.910 They often move to new species over an evolutionary time scale11 and play a major role in dynamic changes to chromosomes during evolution.1213

    The introduction of foreign germplasm into crops has been achieved by traditional crop breeders by artificially overcoming fertility barriers. A hybrid cereal was created in 1875, by crossing wheat and rye.14 Since then important traits have been introduced into wheat, including dwarfing genes and rust resistance.15 Plant tissue culture and the induction of mutations have also enabled humans to artificially alter the makeup of plant genomes.1617


    The first genetically modified plant was produced in 1982, using an antibiotic-resistant tobacco plant.18 The first field trials of genetically engineered plants occurred in France and the USA in 1986, when tobacco plants were engineered to be resistant to herbicides.19 In 1987, Plant Genetic Systems (Ghent, Belgium), founded by Marc Van Montagu and Jeff Schell, was the first company to develop genetically engineered (tobacco) plants with insect tolerance by expressing genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt).20 The People’s Republic of China was the first country to allow commercialized transgenic plants, introducing a virus-resistant tobacco in 1992,21 which was withdrawn from the market in China in 1997.22:3 The first genetically modified crop approved for sale in the U.S., in 1994, was the FlavrSavr tomato, which had a longer shelf life, as it took longer to soften after ripening.23 In 1994, the European Union approved tobacco engineered to be resistant to the herbicide bromoxynil, making it the first commercially genetically engineered crop marketed in Europe.24 In 1995, Bt Potato was approved safe by the Environmental Protection Agency, making it the first pesticide producing crop to be approved in the USA.25 The following transgenic crops also received marketing approval in the US in 1995: canola with modified oil composition (Calgene), Bacillus thuringiensis (Bt) corn/maize (Ciba-Geigy), cotton resistant to the herbicide bromoxynil (Calgene), Bt cotton (Monsanto), soybeans resistant to the herbicide glyphosate (Monsanto), virus-resistant squash (Asgrow), and additional delayed ripening tomatoes (DNAP, Zeneca/Peto, and Monsanto).19 As of mid-1996, a total of 35 approvals had been granted to commercially grow 8 transgenic crops and one flower crop of carnations, with 8 different traits in 6 countries plus the EU.19 In 2000, with the production of golden rice, scientists genetically modified food to increase its nutrient value for the first time.


    Genetically engineered plants are generated in a laboratory by altering their genetic makeup. This is usually done by adding one or more genes to a plant’s genome using genetic engineering techniques.26 Most genetically modified plants are generated by the biolistic method (particle gun) or by Agrobacterium tumefaciens mediated transformation. Plant scientists, backed by results of modern comprehensive profiling of crop composition, point out that crops modified using GM techniques are less likely to have unintended changes than are conventionally bred crops.2728

    In research tobacco and Arabidopsis thaliana are the most genetically modified plants, due to well developed transformation methods, easy propagation and well studied genomes.2930 They serve as model organisms for other plant species.

    In the biolistic method, DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under high pressure. The accelerated particles penetrate both the cell wall and membranes. The DNA separates from the metal and is integrated into plant genome inside the nucleus. This method has been applied successfully for many cultivated crops, especially monocots like wheat or maize, for which transformation using Agrobacterium tumefaciens has been less successful.31 The major disadvantage of this procedure is that serious damage can be done to the cellular tissue.

    Agrobacteria are natural plant parasites, and their natural ability to transfer genes provides another method for the development of genetically engineered plants. To create a suitable environment for themselves, these Agrobacteria insert their genes into plant hosts, resulting in a proliferation of plant cells near the soil level (crown gall). The genetic information for tumour growth is encoded on a mobile, circular DNA fragment (plasmid). When Agrobacterium infects a plant, it transfers this T-DNA to a random site in the plant genome. When used in genetic engineering the bacterial T-DNA is removed from the bacterial plasmid and replaced with the desired foreign gene. The bacterium is a vector, enabling transportation of foreign genes into plants. This method works especially well for dicotyledonous plants like potatoes, tomatoes, and tobacco. Agrobacteria infection is less successful in crops like wheat and maize.

    Introducing new genes into plants requires a promoter specific to the area where the gene is to be expressed. For instance, if we want the gene to be expressed only in rice grains and not in leaves, then an endosperm-specific promoter would be used. The codons of the gene must also be optimized for the organism due to codon usage bias. The transgenic gene products should also be able to be denatured by heat so that they are destroyed during cooking.

    Glyphosate in a human DNA disruptor so why are they spraying anything with Glyphosate????

    Glyphosate resistance

    One of the most famous kinds of GM crops are “Roundup Ready”, or glyphosate-resistant trait. Glyphosate, (the active ingredient in Roundup) kills plants by interfering with the shikimate pathway in plants, which is essential for the synthesis of the aromatic amino acids phenylalanine, tyrosine and tryptophan. More specifically, glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

    The rationale behind developing this trait, was that the selective herbicides used on grain and grass crops at the time were highly toxic, and not effective against narrow leaved weeds. Thus, developing crops that could withstand spraying with glyphosate would both reduce environmental and health risks, and give an agricultural edge to the farmer.32

    The shikimate pathway is not present in animals, which instead obtain aromatic amino acids from their diet.

    Some micro-organisms have a version of EPSPS that is resistant to glyphosate inhibition. One of these was isolated from an Agrobacterium strain CP4 (CP4 EPSPS) that was resistant to glyphosate.3334 This CP4 EPSPS gene was cloned and transfected into soybeans. The CP4 EPSPS gene was engineered for plant expression by fusing the 5′ end of the gene to a chloroplast transit peptide derived from the petunia EPSPS. This transit peptide was used because it had shown previously an ability to deliver bacterial EPSPS to the chloroplasts of other plants. The plasmid used to move the gene into soybeans was PV-GMGTO4. It contained three bacterial genes, two CP4 EPSPS genes, and a gene encoding beta-glucuronidase (GUS) from Escherichia coli as a marker. The DNA was injected into the soybeans using the particle acceleration method. Soybean cultivar A54O3 was used for the transformation. The expression of the GUS gene was used as the initial evidence of transformation. GUS expression was detected by a staining method in which the GUS enzyme converts a substrate into a blue precipitate. Those plants that showed GUS expression were then taken and sprayed with glyphosate, and their tolerance was tested over many generations.

    Types of genetic engineering


    Transgenic plants have genes inserted into them that are derived from another species. The inserted genes can come from species within the same kingdom (plant to plant) or between kingdoms (for example, bacteria to plant). In many cases the inserted DNA has to be modified slightly in order to correctly and efficiently express in the host organism. Transgenic plants are used to express proteins like the cry toxins from Bacillus thuringiensis, herbicide resistant genes and antigens for vaccinations35 Transgenic carrots have been used to produce the drug Taliglucerase alfa which is used to treat Gaucher’s disease.36 In the laboratory, transgenic plants have been modified to increase their photosynthesis (currently about 2% at most plants to the theoretic potential of 9–10%.37 This is possible by changing the rubisco enzyme (i.e. changing C3 plants into C4 plants38), by placing the rubisco in a carboxysome, by adding CO
    2 pumps in the cell wall,3940 by changing the leaf form/size.41424344 Plants have been engineered to exhibit bioluminescence which might one day be a sustainable alternative to electric lighting.45 Still other transgenic plants have been modified to fix ambient nitrogen in the plant.46


    Cisgenic plants are made using genes found within the same species or a closely related one, where conventional plant breeding can occur. Some breeders and scientists argue that cisgenic modification is useful for plants that are difficult to crossbreed by conventional means (such as potatoes), and that plants in the cisgenic category should not require the same level of legal regulation as other genetically modified organisms.47


    In 2014, Chinese researcher Gao Caixia filed patents on the creation of a strain of wheat that is resistant to powdery mildew. The strain lacks genes that encode proteins that repress defenses against the mildew. The researchers deleted all three copies of the genes from wheat’s hexaploid genome. The strain promises to reduce or eliminate the heavy use of fungicides to control the disease. Gao used the TALENs and CRISPR gene editing tools without adding or changing any other genes. No field trials are yet planned.4849

    Business of GM Crops

    The global value of biotech seed alone was US$13.2 billion in 2011, with the end product of commercial grain from biotech maize, soybean grain and cotton valued at approximately US$160 billion or more per year.50

    Players in agriculture business markets include seed companies, agrochemical companies, distributors, farmers, grain elevators, and universities that develop new crops and whose agricultural extensions advise farmers on best practices.

    The largest share of the GMO crops planted globally are from seed created by the United States firm Monsanto.51 In 2007, Monsanto’s trait technologies were planted on 246 million acres (1,000,000 km2) throughout the world, a growth of 13 percent from 2006. However, patents on the first Monsanto products to enter the marketplace will begin to expire in 2014, democratizing Monsanto products. Syngenta, DuPont (especially via its Pioneer Hi-Bred subsidiary), and Bayer CropScience are also major players in the US and Europe. In addition, a 2007 report from the European Joint Research Commission predicts that by 2015, more than 40 per cent of new GM plants entering the global marketplace will have been developed in Asia.52

    In the corn market, Monsanto’s triple-stack corn—which combines Roundup Ready 2-weed control technology with YieldGard (Bt) Corn Borer and YieldGard Rootworm insect control—is the market leader in the United States. U.S. corn farmers planted more than 32 million acres (130,000 km2) of triple-stack corn in 2008,53 and it is estimated the product could be planted on 56 million acres (230,000 km2) in 2014–2015. In the cotton market, Bollgard II with Roundup Ready Flex was planted on approximately 5 million acres (20,000 km2) of U.S. cotton in 2008.54

    According to the International Service for the Acquisition of Agri-Biotech Applications (ISAAA), in 2010 approximately 15 million farmers grew biotech crops in 29 countries. Over 90% of the farmers were resource-poor in developing countries.55 6.5 million farmers in China and 6.3 million small farmers in India grew biotech crops (mostly Bacillus thuringiensis cotton). The Philippines, South Africa (biotech cotton, maize, and soybeans often grown by subsistence women farmers) and another twelve developing countries also grew biotech crops in 2009.56 10 million more small and resource-poor farmers may have been secondary beneficiaries of Bt cotton in China.

    According to a review published in 2012 and based on data from the late 1990s and early 2000s, much of the GM crop grown each year is used for livestock feed, and increased demand for meat will lead to increased demand for GM crops with which to feed them.57 Feed grain usage as a percentage of total crop production is 70% for corn and more than 90% of oil seed meals such as soybeans. About 65 million metric tons of GM corn grains and about 70 million metric tons of soybean meals derived from GM soybean are fed to livestock each year.57

    Uses, actual and proposed

    GM crops grown today, or under experimental development, have been modified with traits intended to provide benefit to farmers, consumers, or industry. These traits include improved shelf life, disease resistance, stress resistance, herbicide resistance, pest resistance, production of useful goods such as biofuel or drugs, and ability to absorb toxins, for use in bioremediation of pollution. Due to high regulatory and research costs, the majority of genetically modified crops in agriculture consist of commodity crops, such as soybean, maize, cotton and rapeseed.5859 Recently, some research and development has been targeted to enhancement of crops that are locally important in developing countries, such as insect-resistant cowpea for Africa60 and insect-resistant brinjal (eggplant) for India.61

    Improved shelf life

    The first genetically modified crop approved for sale in the U.S. was the FlavrSavr tomato, which had a longer shelf life.23 It is no longer on the market. As of 2013, an apple that has been genetically modified to resist browning, known as the Nonbrowning Arctic apple produced by Okanagan Specialty Fruits, is awaiting regulatory approval in the US and Canada. A gene in the fruit has been modified such that the apple produces less polyphenol oxidase, a chemical that manifests the browning.62 If approved by U.S. regulators in the coming months, they will be one of the first genetically engineered fruits on store shelves in America.63

    Stress resistance

    Plants engineered to tolerate non-biological stresses like drought,7273 frost,7475 high soil salinity,7677 and nitrogen starvation78 or with increased nutritional value (e.g. Golden rice79) were in development in 2011. In 2011, Monsanto’s DroughtGard became the first drought resistant GM crop – a genetically modified maize to receive marketing approval in the US.80

    Herbicide resistance

    One of the most prevalent type of GM crops have a “Roundup Ready”, or glyphosate-resistant trait.32 Tobacco plants have been engineered to be resistant to the herbicide bromoxynil.24 Crops have been commercialized that are resistant to the herbicide glufosinate, as well.81 As weeds have grown resistant to glyphosate and other herbicides used in concert with resistant GM crops, companies are developing crops engineered to become resistant to multiple herbicides to allow farmers to use a mixed group of two, three, or four different chemicals.8283

    Pathogen resistance

    Tobacco, corn, rice and many other crops, have been generated that express genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt).2584 Papaya, potatoes, and squash have been engineered to resist viral pathogens, such as cucumber mosaic virus which, despite its name, infects a wide variety of plants.85 As of 2013, trials are underway on genetically modified oranges that can resist citrus greening disease.

    Production of biofuels

    Algae, both hybrid and GM, is under development by several companies for the production of biofuels.86 Jatropha has also been modified to improve its qualities for fuel product. Swiss-based Syngenta has received USDA approval to market a maize seed trademarked Enogen, which has been genetically modified to convert its own starch to sugar to speed the process of making ethanol for biofuel.87 In 2013, the Flemish Institute for Biotechnology was investigating poplar trees genetically engineered to contain less lignin so that they would be more suitable for conversion into biofuels.88 Lignin is the critical limiting factor when using wood to make bio-ethanol because lignin limits the accessibility of cellulose microfibrils to depolymerization by enzymes.89

    Production of useful by-products


    In 2012, the FDA approved the first plant-produced pharmaceutical, a treatment for Gaucher’s Disease.90 Tobacco plants have been developed and studied, but are not in production, that can produce therapeutic antibodies.91


    Bananas have been developed that produce human vaccines against infectious diseases such as Hepatitis B.92 Another example is the expression of a fusion protein in alfalfa transgenic plants for the selective directioning to antigen presenting cells, therefore increasing vaccine potency against Bovine Viral Diarrhea Virus (BVDV).9394


    Several companies and labs are working on engineering plants that can be used to make bioplastics.95 Potatoes that produce more industrially useful starches have been developed as well.96 Additionally, oilseed can be modified to produce fatty acids for detergents, substitute fuels and petrochemicals.


    Scientists at the University of York developed a weed (Arabidopsis thaliana) that contains genes from bacteria that can clean up TNT and RDX-explosive contaminants from the soil: It was hoped that this weed would eliminate this pollution.97 16 million hectares in the USA (1.5% of the total surface) are estimated to be contaminated with TNT and RDX. However the weed Arabidopsis thaliana was not tough enough to withstand the environment on military test grounds and research is continuing with the University of Washington to develop a tougher native grass.98

    Genetically modified plants have also been used for bioremediation of contaminated soils. Mercury, selenium and organic pollutants such as polychlorinated biphenyls (PCBs), TNT and RDX explosive contaminants have been removed from soils by transgenic plants containing genes for bacterial enzymes.9899

    Marine environments are especially vulnerable since oil spills of coastal regions and the open sea are poorly containable and mitigation is difficult. In addition to pollution through human activities, millions of tons of petroleum enter the marine environment every year from natural seepages. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbon-degrading activities of microbial communities. Particularly successful is a recently discovered group of specialists, the so-called hydrocarbonoclastic bacteria (HCCB).100

    Managing emergence of resistance

    Constant exposure to a toxin creates evolutionary pressure for pests resistant to that toxin.

    One method of reducing resistance is the creation of non-Bt crop refuges to allow some nonresistant insects to survive and maintain a susceptible population. To reduce the chance an insect would become resistant to a Bt crop, the commercialization of transgenic cotton and maize in 1996 was accompanied with a management strategy to prevent insects from becoming resistant to Bt crops, and insect resistance management plans are mandatory for Bt crops planted in the USA and other countries. The aim is to encourage a large population of pests so that any resistance genes that are recessive are greatly diluted within the population.136

    This means that with sufficiently high levels of transgene expression, nearly all of the heterozygotes (S/s), i.e., the largest segment of the pest population carrying a resistance allele, will be killed before they reach maturity, thus preventing transmission of the resistance gene to their progeny.137 The planting of refuges (i. e., fields of nontransgenic plants) adjacent to fields of transgenic plants increases the likelihood that homozygous resistant (s/s) individuals and any surviving heterozygotes will mate with susceptible (S/S) individuals from the refuge, instead of with other individuals carrying the resistance allele. As a result, the resistance gene frequency in the population would remain low.

    Nevertheless, limitations can affect the success of the high-dose/refuge strategy. For example, expression of the Bt gene can vary. For instance, if the temperature is not ideal, this stress can lower the toxin production and make the plant more susceptible. More importantly, reduced late-season expression of toxin has been documented, possibly resulting from DNA methylation of the promoter.138 So, while the high-dose/refuge strategy has been successful at prolonging the durability of Bt crops, this success has also had much to do with key factors independent of management strategy, including low initial resistance allele frequencies, fitness costs associated with resistance, and the abundance of non-Bt host plants that have supplemented the refuges planted as part of the resistance management strategy.139

    Companies that produce Bt seed are addressing this as well, by introducing plants with multiple Bt proteins. Monsanto did this with Bt cotton in India, where the product was rapidly adopted.140


    The regulation of genetic engineering concerns the approaches taken by governments to assess and manage the risks associated with the development and release of genetically modified crops. There are differences in the regulation of GM crops between countries, with some of the most marked differences occurring between the USA and Europe. Regulation varies in a given country depending on the intended use of the products of the genetic engineering. For example, a crop not intended for food use is generally not reviewed by authorities responsible for food safety.141142


    The genetically modified foods controversy is a dispute over the use of food and other goods derived from genetically modified crops instead of from conventional crops, and other uses of genetic engineering in food production. The dispute involves consumers, biotechnology companies, governmental regulators, non-governmental organizations, and scientists. The key areas of controversy related to genetically modified food are: whether GM food should be labeled, the role of government regulators, the effect of GM crops on health and the environment, the effect on pesticide resistance, the impact of GM crops for farmers, and the role of GM crops in feeding the world population.

    There is broad scientific consensus that food on the market derived from GM crops poses no greater risk than conventional food.13143 No reports of ill effects have been documented in the human population from GM food.4144145 Although labeling of genetically modified organism (GMO) products in the marketplace is required in many countries, in the United States, the Food and Drug Administration does not require labeling of GMO products in the marketplace, nor does it recognize a distinction between GMO and non-GMO foods.146

    Some advocacy groups such as Greenpeace and the World Wildlife Fund have concerns that risks of GM food have not been adequately identified and managed, and have questioned the objectivity of regulatory authorities. Other environmental groups, including The Nature Conservancy147 and former anti-GMO campaigner Mark Lynas support the use of GMOs as beneficial for the environment.148


    American Association for the Advancement of Science (AAAS), Board of Directors (2012). Legally Mandating GM Food Labels Could Mislead and Falsely Alarm Consumers

    A decade of EU-funded GMO research (2001-2010) (PDF). Directorate-General for Research and Innovation. Biotechnologies, Agriculture, Food. European Union. 2010. doi:10.2777/97784. ISBN 978-92-79-16344-9. “”The main conclusion be drawn from the efforts of more than 130 research projects, covering a period of more than 25 years of research, and involving more than 500 independent research groups, is that biotechnology, and in particular GMOs, are not per se more risky than e.g. conventional plant breeding technologies.” (p. 16)”

    : a b Ronald, Pamela (2011). “Plant Genetics, Sustainable Agriculture and Global Food Security”. Genetics 188 (1): 11–20. doi:10.1534/genetics.111.128553.

    : a b American Medical Association (2012). Report 2 of the Council on Science and Public Health: Labeling of Bioengineered Foods

    FAO, 2004. State of Food and Agriculture 2003–2004. Agricultural Biotechnology: Meeting the Needs of the Poor. Food and Agriculture Organization of the United Nations, Rome. “Currently available transgenic crops and foods derived from them have been judged safe eat and the methods used test their safety have been deemed appropriate. These conclusions represent the consensus of the scientific evidence surveyed by the ICSU (2003) and they are consistent with the views of the World Health Organization (WHO, 2002). These foods have been assessed for increased risks human health by several national regulatory authorities (inter alia, Argentina, Brazil, Canada, China, the United Kingdom and the United States) using their national food safety procedures (ICSU). date no verifiable untowardxic or nutritionally deleterious effects resulting from the consumption of foods derived from genetically modified crops have been discovered anywhere in the world (GM Science Review Panel). Many millions of people have consumed foods derived from GM plants – mainly maize, soybean and oilseed rape – without any observed adverse effects (ICSU).”

    Andrew Pollack for the New York Times. April 13, 2010 Study Says Overuse Threatens Gains From Modified Crops

    Lederberg J, Tatum EL (1946). “Gene recombination in E. coli”. Nature 158 (4016): 558. Bibcode:1946Natur.158..558L. doi:10.1038/158558a0.

    Bock, R. (2010). “The give-and-take of DNA: horizontal gene transfer in plants”. Trends in Plant Science 15 (1): 11–22. doi:10.1016/j.tplants.2009.10.001. PMID 19910236. edit

    Morgante, M.; Brunner, S.; Pea, G.; Fengler, K.; Zuccolo, A.; Rafalski, A. (2005). “Gene duplication and exon shuffling by helitron-like transposons generate intraspecies diversity in maize”. Nature Genetics 37 (9): 997–1002. doi:10.1038/ng1615. PMID 16056225. edit

    Monroe D. (2006). “Jumping Genes Cross Plant Species Boundaries”. PLoS Biology 4 (1): e35. doi:10.1371/journal.pbio.0040035. edit

    Feschotte, C.; Osterlund, M. T.; Peeler, R.; Wessler, S. R. (2005). “DNA-binding specificity of rice mariner-like transposases and interactions with Stowaway MITEs”. Nucleic Acids Research 33 (7): 2153. doi:10.1093/nar/gki509. PMC 1079968. PMID 15831788. edit

    Cordaux, R.; Udit, S.; Batzer, M.; Feschotte, C. (2006). “Birth of a chimeric primate gene by capture of the transposase gene from a mobile element”. Proceedings of the National Academy of Sciences of the United States of America 103 (21): 8101–8106. Bibcode:2006PNAS..103.8101C. doi:10.1073/pnas.0601161103. PMC 1472436. PMID 16672366. edit

    Long, M.; Betrán, E.; Thornton, K.; Wang, W. (2003). “The origin of new genes: glimpses from the young and old”. Nature reviews. Genetics 4 (11): 865–875. doi:10.1038/nrg1204. PMID 14634634. edit

    Chen, Z. (2010). “Molecular mechanisms of polyploidy and hybrid vigor”. Trends in Plant Science 15 (2): 57–71. doi:10.1016/j.tplants.2009.12.003. PMC 2821985. PMID 20080432. edit

    Hoisington D, Khairallah M, Reeves T, Ribaut JM, Skovmand B, Taba S, Warburton M (1999). “Plant genetic resources: What can they contributeward increased crop productivity?”. Proceedings of the National Academy of Sciences of the United States of America 96 (11): 5937–43. Bibcode:1999PNAS…96.5937H. doi:10.1073/pnas.96.11.5937. ISSN 0027-8424. PMC 34209. PMID 10339521.

    Predieri, S. (2001). Plant Cell, Tissue and Organ Culture 64 (2/3): 185–210. doi:10.1023/A:1010623203554. edit

    Duncan, R. (1996). Tissue Culture-Induced Variation and Crop Improvement 58. pp. 201–200. doi:10.1016/S0065-2113(08)60256-4. edit

    Fraley, RT et al. (1983) Expression of bacterial genes in plant cells. Proc. NatL. Acad. Sci. USA 80: 4803–4807 1

    : a b c James, Clive (1996). “Global Review of the Field Testing and Commercialization of Transgenic Plants: 1986 1995”. The International Service for the Acquisition of Agri-biotech Applications. Retrieved 17 July 2010.

    Vaeck, M et al. (1987) Transgenic plants protected from insect attack. Nature 328, 33–37 Transgenic plants protected from insect attack 2

    James, C (1997). “Global Status of Transgenic Crops in 1997”. ISAAA Briefs No. 5.: 31.

    Conner AJ, Glare TR, Nap JP. The release of genetically modified crops into the environment. Part II. Overview of ecological risk assessment Plant J. 2003 Jan;33(1):19-46.

    : a b Bruening, G.; Lyons, J. M. (2000). “The case of the FLAVR SAVRmato”. California Agriculture 54 (4): 6–7. doi:10.3733/ca.v054n04p6. edit

    : a b Debora MacKenzie (18 June 1994). “Transgenicbacco is European first”. New Scientist.

    : a b Genetically Altered Potato Ok’d For Crops Lawrence Journal-World – 6 May 1995

    Rebecca Boyle for Popular Science. January 24, 2011. How Genetically Modify a Seed, Step By Step

    Proteomic profiling and unintended effects in genetically modified crops, Sirpa O. Kärenlampi and Satu J. Lehesranta 2006

    Catchpole, G. S. (2005). “Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops”. Proceedings of the National Academy of Sciences 102 (40): 14458. Bibcode:2005PNAS..10214458C. doi:10.1073/pnas.0503955102.

    Koornneef, M.; Meinke, D. (2010). “The development of Arabidopsis as a model plant”. The Plant journal : for cell and molecular biology 61 (6): 909–921. doi:10.1111/j.1365-313X.2009.04086.x. PMID 20409266. edit

    “Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut improve saltlerance – Springer”. Link.springer.com. 2012-01-01. Retrieved 2013-12-19.

    Shrawat, A.; Lörz, H. (2006). “Agrobacterium-mediated transformation of cereals: a promising approach crossing barriers”. Plant biotechnology journal 4 (6): 575–603. doi:10.1111/j.1467-7652.2006.00209.x. PMID 17309731. edit

    : a b Carpenter J. & Gianessi L. (1999). Herbicidelerant soybeans: Why growers are adopting Roundup Ready varieties. AgBioForum, 2(2), 65-72.

    G. R. Heck, et al. (1 January 2005). “Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event” (Free full text). Crop Sci. 45 (1): 329–339. doi:10.2135/cropsci2005.0329.

    T. Funke et al. (2006). “Molecular basis for the herbicide resistance of Roundup Ready crops” (Free full text). PNAS 103 (35): 13010–13015. Bibcode:2006PNAS..10313010F. doi:10.1073/pnas.0603638103. PMC 1559744. PMID 16916934.

    Walmsley, A.; Arntzen, C. (2000). “Plants for delivery of edible vaccines”. Current Opinion in Biotechnology 11 (2): 126. doi:10.1016/S0958-1669(00)00070-7. PMID 10753769. edit

    Maxmen, Amy (2 May 2012) First plant-made drug on the market Nature, Biology & Biotechnology, Industry. Retrieved 26 June 2012

    NWT magazine, april 2011

    Project at IRRI making C3 into C4 plants

    Project by Dean Price increasing photosynthesis by 15 25%

    Additional project by Dean Price; adding of CO²-concentrating cage

    Project by Gerrit Beemster changing leaf size

    Project by Neelima Sinha changing leaf shape

    Projects changing respectively plant growth and plant flowers

    Project changing number of stomata in plants conducted by Ikuko Hara-Nishimura

    (4 May 2013) One Per Cent: Grow your own living lights The New Scientist, Issue 2915, Retrieved 7 May 2013

    Project by Andreas Weber described at The Plant Journal, january 2011

    MacKenzie, Deborah (2 August 2008). “How the humble potato could feed the world” (cover story) New Scientist. No 2667 pp.30–33

    Talbot, David (2014-07-19). “Beijing Researchers Use Gene Editing Create Disease-Resistant Wheat | MIT Technology Review”. Technologyreview.com. Retrieved 2014-07-23.

    . doi:10.1038/nbt.2969. Missing or empty |title= (help) edit

    : a b c d e f g h James, C (2011). “ISAAA Brief 43, Global Status of Commercialized Biotech/GM Crops: 2011”. ISAAA Briefs. Ithaca, New York: International Service for the Acquisition of Agri-biotech Applications (ISAAA). Retrieved 2012-06-02.

    Company Profile Monsanto. Retrieved 6 August 2010.

    Alexander J. Stein and Emilio Rodríguez-Cerezo (2009) The global pipeline of new GM crops, Implications of asynchronous approval for international trade. Institute for Prospective Technological Studies of the European Commission Joint Research Centre. Retrieved 18 January 2011

    Kaskey, Jack Monsanto, Dow Chemical Win Approval for Modified Corn (Update3) Bloomberg News, 20 July 2009. Retrieved 10 August 2010.

    Monsanto’s Bollgard II with Roundup Ready Flex cotton delivers positive results in 2008 7 January 2009. Retrieved 10 August 2010.

    James, Clive (2010) Global Status of Commercialized Biotech/GM Crops: 2010 ISAAA Brief No. 42. ISAAA: Ithaca, NY. Retrieved 10 October 2011

    Global Status of Commercialized Biotech/GM Crops: 2009 The first fourteen years, 1996 2009 ISAAA Brief 41-2009, 23 February 2010. Retrieved 10 August 2010.

    : a b Rajib Deb, et al. “Genetically Modified (Gm) Crops Lifeline For Livestock – A Review.” Agricultural Reviews 31.4 (2010): 279–285.

    Qaim, Matin The Benefits of Genetically Modified Crops—and the Costs of Inefficient Regulation Resources for the Future, 2 April 2010. Retrieved 11 August 2010.

    Field areas 2009: Genetically modified plants: Global cultivation on 134 million hectares GMO Compass, 29 March 2010. Retrieved 11 August 2010.

    CSIRO 2006 press release on SeedQuest website

    ISAAA Pocket K No. 35: Bt Brinjal in India

    Haroldsen, Victor M.; Paulino, Gabriel; Chi-ham, Cecilia; Bennett, Alan B. (2012). “Research and adoption of biotechnology strategies could improve California fruit and nut crops”. California Agriculture 66 (2): 62–69. doi:10.3733/ca.v066n02p62.

    Iritani, Evelyn. “Who’s Banking on GMO Apples?”. The Huffington Post. Retrieved 24 October 2013.

    Canadian Food Inspection Agency. DD2009-76: Determination of the Safety of Pioneer Hi-Bred Production Ltd.’s Soybean (Glycine max (L.) Merr.) Event 305423 Issued: 2009-04 3. Retrieved January 2011

    Andrew Pollack for the New York Times. November 15, 2013 In a Bean, a Boon Biotech

    http://dtma.cimmyt.org. Retrieved 21 January 2011

    Sayre, R.; Beeching, J. R.; Cahoon, E. B.; Egesi, C.; Fauquet, C.; Fellman, J.; Fregene, M.; Gruissem, W.; Mallowa, S.; Manary, M.; Maziya-Dixon, B.; Mbanaso, A.; Schachtman, D. P.; Siritunga, D.; Taylor, N.; Vanderschuren, H.; Zhang, P. (2011). “The BioCassava Plus Program: Biofortification of Cassava for Sub-Saharan Africa”. Annual Review of Plant Biology 62: 251–272. doi:10.1146/annurev-arplant-042110-103751. PMID 21526968. edit

    About Golden Rice International Rice Research Institute. Retrieved 20 August 2012

    Shaista Naqvi, et al. Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways PNAS April 27, 2009.

    Crop plants – “green factories” for fish oils, Rothamsted Research 14-11-2013.

    Successful high-level accumulation of fish oil omega-3 long chain polyunsaturated fatty acids in a transgenic oilseed crop, Ruiz-Lopez, Noemi et al., The Plant Journal, accepted article, DOI:10.1111/tpj.12378, 2013.

    Paarlburg, Robert Droughtlerant GMO Maize in Africa, Anticipating Regulatory Hurdles International Life Sciences Institute, January 2011. Retrieved 25 April 2011

    Australia continues test drought-resistant GM wheat GMO Compass, 16 July 2008. Retrieved 25 April 2011

    Staff (14 May 2011) USA: USDA allows large-scale GM eucalyptus trial GMO Cmpass. Retrieved 29 September 2011Lundmark, C. (2006). “Searching Evolutionary Pathways: Antifreeze Genes from Antarctic Hairgrass”. BioScience 56 (6): 552. doi:10.1641/0006-3568(2006)56552:SEP2.0.CO;2. ISSN 0006-3568. edit

    Banjara, Manoj; Longfu Zhu; Guoxin Shen; Paxton Payton; Hong Zhang (2012). “Expression of an Arabidopsis sodium/proton antiporter gene ( AtNHX1 ) in peanut to improve salt tolerance”. Plant Biotechnol Rep 6. doi:10.1007/s11816-011-0200-5.

    Sara Abdulla (27 May 1999). “Drought stress”. Nature News. doi:10.1038/news990527-9.

    Rennie, Rob and Heffer, Patrick Anticipated Impact of Modern Biotechnology on Nutrient Use Efficiency TFI/FIRT Fertilizer Outlook and Technology Conference 16–18 November 2010, Savannah (GA), Web page. Retrieved 25 April 2011

    Nayar, A. (2011). “Grants aim to fight malnutrition”. Nature. doi:10.1038/news.2011.233. edit

    Michael Eisenstein Plant breeding: Discovery in a dry spell Nature 501, S7–S9 (26 September 2013) Published online 25 September 2013

    L. P. Gianessi, C. S. Silvers, S. Sankula and J. E. Carpenter. Plant Biotechnology: Current and Potential Impact for Improving Pest management in US Agriculture, An Analysis of 40 Case Studies (Washington, D.C.: National Center for Food and Agricultural Policy, 2002), 5–6

    Kasey J. (8 September 2011)Attack of the Superweed. Bloomberg Businessweek.

    Mark Ganchiff for Midwest Wine Press. August 24, 2013 New Herbicide Resistant Crops Being Considered By USDA

    Vaeck, M.; Reynaerts, A.; Höfte, H.; Jansens, S.; De Beuckeleer, M.; Dean, C.; Zabeau, M.; Montagu, M. V.; Leemans, J. (1987). “Transgenic plants protected from insect attack”. Nature 328 (6125): 33. Bibcode:1987Natur.328…33V. doi:10.1038/328033a0. edit

    National Academy of Sciences (2001). Transgenic Plants and World Agriculture. Washington: National Academy Press.

    Carrington, Damien (19 January 2012) GM microbe breakthrough paves way for large-scale seaweed farming for biofuels The Guardian. Retrieved 12 March 2012

    Carolyn Lochhead for the San Francisco Chronicle. 30 April 2012 Genetically modified crops’ results raise concern

    Hope, Alan (3 April 2013, News in brief: The Bio Safety Council…” Flanders Today, Page 2, Retrieved 27 April 2013

    (2013) Wout Boerjan Lab VIB (Flemish Institute for Biotechnology) Gent, Retrieved 27 April 2013

    Gali Weinreb and Koby Yeshayahou for Globes May 2, 2012. FDA approves Protalix Gaucher treatment

    Jha, Alok (14 August 2012) Julian Ma: I’m growing antibodies in tobacco plants to help prevent HIV The Guardian. Retrieved 12 March 2012

    Kumar, G. B. Sunil; T. R. Ganapathi, C. J. Revathi, L. Srinivas and V. A. Bapat (October 2005). “Expression of hepatitis B surface antigen in transgenic banana plants”. Planta 222 (3): 484–493. doi:10.1007/s00425-005-1556-y. PMID 15918027.

    Ostachuk A; Chiavenna SM; Gómez C; Pecora A; Pérez-Filgueira MD; Escribano JA; Ardila F; Dus Santos MJ et al. (2009). “Expression of a ScFv–E2T fusion protein in CHO-K1 cells and alfalfa transgenic plants for the selective directioning to antigen presenting cells”. Veterinary Immunology and Immunopathology 128 (1): 315.

    Aguirreburualde MSP; Gómez C; Ostachuk A; Wolman F; Albanesi G; Pecora A; Odeon A; Ardila F; Escribano JA; Dus Santos MJ; Wigdorovitz A (2013). “Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants”. Veterinary Immunology and Immunopathology 151 (3): 315–324.

    van Beilen, Jan B.; Yves Poirier (May 2008). “Harnessing plant biomass for biofuels and biomaterials:Production of renewable polymers from crop plants”. The Plant Journal 54 (4): 684–701. doi:10.1111/j.1365-313X.2008.03431.x. PMID 18476872.

    a b “The History and Future of GM Potatoes”. PotatoPro Newsletter. March 10, 2010.

    Strange, Amy (20 September 2011) Scientists engineer plants to eat toxic pollution The Irish Times. Retrieved 20 September 2011

    a b Chard, Abigail (2011) Growing a grass that loves bombs The British Science Association. Retrieved 20 September 2011

    Meagher, RB (2000). “Phytoremediation of toxic elemental and organic pollutants”. Current Opinion in Plant Biology 3 (2): 153–162. doi:10.1016/S1369-5266(99)00054-0. PMID 10712958.

    Martins VAP (2008). “Genomic Insights into Oil Biodegradation in Marine Systems”. Microbial Biodegradation: Genomics and Molecular Biology. Caister Academic Press. ISBN 978-1-904455-17-2.

    a b ISAAA 2012 Annual Report Executive Summary

    Fernandez-Cornejo, Jorge (1 July 2009). Adoption of Genetically Engineered Crops in the U.S.. Data Sets. Economic Research Service, United States Department of Agriculture. OCLC 53942168. Archived from the original on 24 September 2009. Retrieved 24 September 2009.

    James, Clive (2007). “Executive Summary”. G lobal Status of Commercialized Biotech/GM Crops: 2007. ISAAA Briefs 37. The International Service for the Acquisition of Agri-biotech Applications (ISAAA). ISBN 978-1-892456-42-7. OCLC 262649526. Archived from the original on 24 September 2009. Retrieved 24 September 2009.

    a b “Roundup Ready soybean trait patent nears expiration in 2014”. Hpj.com. Retrieved 2013-12-19.

    Adoption of Genetically Engineered Crops in the U.S. – Economic Research Service, of the U.S. Department of Agriculture

    a b c Acreage NASS National Agricultural Statistics Board annual report, 30 June 2010. Retrieved 23 July 2010.

    a b USA:Cultivation of GM Plants in 2009, Maize, soybean, cotton: 88 percent genetically modified GMO Compass. Retrieved 25 July 2010.

    Fernandez-Cornejo, Jorge (5 July 2012) Adoption of Genetically Engineered Crops in the U.S. – Recent Trends USDA Economic Research Service. Retrieved 29 September 2012

    Genetic Engineering: The Future of Foods?

    Lemaux, Peggy (19 February 2008). “Genetically Engineered Plants and Foods: A Scientist’s Analysis of the Issues (Part I)”. Annual Review of Plant Biology 59: 771–812. doi:10.1146/annurev.arplant.58.032806.103840. PMID 18284373. Retrieved 9 May 2009.

    Spain, Bt maize prevails GMO Compass, 31 March 2010. Retrieved 10 August 2010.

    GM plants in the EU in 2009 Field area for Bt maize decreases GMO Compass, 29 March 2010. Retrieved 10 August 2010.

    “EU GMO ban was illegal, WTO rules”. Euractiv.com. 12 May 2006. Retrieved 5 January 2010.

    “GMO Update: US-EU Biotech Dispute; EU Regulations; Thailand”. International Centre for Trade and Sustainable Development. Retrieved 5 January 2010.

    “Genetically Modified Food and Feed”. Retrieved 5 January 2010.

    Field areas 2009, Genetically modified plants: Global cultivation on 134 million hectares GMO Compass. Retrieved 25 July 2010.

    Ronald, Pamela and McWilliams, James Genetically Engineered Distortions The New York Times, 14 May 2010, Mentions that “in the early 1990s, Hawaii’s papaya industry was facing disaster because of the deadly papaya ringspot virus. Its single-handed savior was a breed engineered to be resistant to the virus. Without it, the state’s papaya industry would have collapsed. Today, 80 percent of Hawaiian papaya is genetically engineered, and there is still no conventional or organic method to control ringspot virus.” Retrieved 26 July 2010.

    Wright, Brierley How Healthy Is Canola Oil Really? “Eating Well”, March/April 2010 edition, Mentions 93% of rapeseed in the US is GM. Retrieved 26 July 2010.

    a b c Johnson, Stanley R. et al Quantification of the Impacts on US Agriculture of Biotechnology-Derived Crops Planted in 2006 National Center for Food and Agricultural Policy, Washington DC, February 2008. Retrieved 12 August 2010.

    Rapeseed (canola) has been genetically engineered to modify its oil content with a gene encoding a “12:0 thioesterase” (TE) enzyme from the California bay plant (Umbellularia californica) to increase medium length fatty acids, see: Geo-pie.cornell.edu

    a b Pocket K No. 2: Plant Products of Biotechnology ISAAA, August 2009. Retrieved 8 September 2012.

    Pollack, Andrew (11 February 2011). U.S. Approves Corn Modified for Ethanol, New York Times

    For a list of all traits, see table at National Corn Growers Association website As of September 2012 that site listed 13 traits in nearly 30 different products.

    Richard M. Manshardt ‘UH Rainbow’ Papaya: A High-Quality Hybrid with Genetically Engineered Disease Resistance. Cooperative Extension Service/CTAHR, University of Hawaii at Manoa.

    Staff, CERA. NewLeaf Entry in CERA

    James Kanter for the New York Times. January 16, 2012. BASF to Stop Selling Genetically Modified Products in Europe

    International Rice Research Institute website: About Golden Rice

    (2014) The Science of Golden Rice The Golden Rice Project, Retrieved 15 March 2014

    Spady, Tyrone (January/February 2014) Pope Blesses Golden Rice ASBP News, Volume 41, Number 1, Page 11, Retrieved 2 March 2014

    Melody M. Bomgardner (2012) Replacing Trans Fat: New crops from Dow Chemical and DuPont target food makers looking for stable, heart-healthy oils. Chemical and Engineering News 90(11):30–32 4

    APHIS Deregulation documents

    CERES database – Squash entry

    Lee SL et al (2012) Pollen allergic risk assessment of genetically modified virus resistant pepper and functional Chinese cabbage” Horticulture, Environment, and Biotechnology 53(2): 167–174, DOI: 10.1007/s13580-012-0092-5 5

    Paroda, Raj (Secretary) Biosafety Regulations of Asia-Pacific Countries FAO, APCoAB, APAARI, 2008. Retrieved 2 September 2012.

    U.S. Food and Drug Administration Center for Food Safety and Applied Nutrition, Biotechnology of Food. FDA Backgrounder: May 18, 1994.

    Insect Resistance to Transgenic Bt Crops: Lessons from the Laboratory and Field, Tabashnik et al. J. Econ. Entomol. 96(4): 1031Ð1038 (2003)

    Roush RT (1997). “Bt-transgenic crops: just another pretty insecticide or a chance for a new start in the resistance management?”. Pestic. Sci. 51 (3): 328–34. doi:10.1002/(SICI)1096-9063(199711)51:3<328::AID-PS650>3.0.CO;2-B.

    Dong, H. Z.; Li, W. J. (2007). “Variability of Endotoxin Expression in Bt Transgenic Cotton”. Journal of Agronomy & Crop Science 193: 21–9. doi:10.1111/j.1439-037X.2006.00240.x.

    Tabashnik BE, Carrière Y, Dennehy TJ (August 2003). “Insect resistance to transgenic Bt crops: lessons from the laboratory and field”. J. Econ. Entomol. 96 (4): 1031–8. doi:10.1603/0022-0493-96.4.1031. PMID 14503572.

    Monsanto website on Bt cotton in India

    Wesseler, J. and N. Kalaitzandonakes (2011): Present and Future EU GMO policy. In Arie Oskam, Gerrit Meesters and Huib Silvis (eds.), EU Policy for Agriculture, Food and Rural Areas. Second Edition, pp. 23-323 – 23-332. Wageningen: Wageningen Academic Publishers

    Beckmann, V., C. Soregaroli, J. Wesseler (2011): Coexistence of genetically modified (GM) and non-modified (non GM) crops: Are the two main property rights regimes equivalent with respect to the coexistence value? In “Genetically modified food and global welfare” edited by Colin Carter, GianCarlo Moschini and Ian Sheldon, pp 201-224. Volume 10 in Frontiers of Economics and Globalization Series. Bingley, UK: Emerald Group Publishing

    Bett, Charles; Ouma, James Okuro; Groote, Hugo De (August 2010). “Perspectives of gatekeepers in the Kenyan food industry towards genetically modified food”. Food Policy 35 (4): 332–340. doi:10.1016/j.foodpol.2010.01.003.

    United States Institute of Medicine and National Research Council (2004). Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects. National Academies Press. Free full-text. National Academies Press. See pp11ff on need for better standards and tools to evaluate GM food.

    Key S, Ma JK, Drake PM (June 2008). “Genetically modified plants and human health”. J R Soc Med 101 (6): 290–8. doi:10.1258/jrsm.2008.070372. PMC 2408621. PMID 18515776.

    Andrew Pollack for the New York Times. “An Entrepreneur Bankrolls a Genetically Engineered Salmon” Published: May 21, 2012. Accessed September 3, 2012

    Could Conservation-Friendly Farming Include GMOs?, by Peter Kareiva, Conservancy Talk, August 11, 2012.

    Biotechnology: Answers to Common Questions, by Kevin Keener, North Carolina State University, June 16, 2013.

TruthWiki © 2017. All Rights Reserved.
Powered by TruthWiki.